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https://doi.org/10.17504/proto...
Article . 2018 . Peer-reviewed
License: CC BY
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Enterovirus (EV) D68 TaqMan 2018 (EV-D68-TM2018) v1

descriptionPublicationkeyboard_double_arrow_right Article 06 Nov 2018Publisher:Springer Science and Business Media LLC
Authors: Ian M Mackay; Judy A Northill;

doi: 10.17504/protocols.io.vche2t6

Enterovirus (EV) D68 TaqMan 2018 (EV-D68-TM2018) v1

Abstract

This protocol aims to amplify enterovirus (EV) D68 viruses and not other viruses. This protocol is modified from a previously published method cited below. Details of the modification are included in the method. The oligonucleotides target the 5'UTR noncoding region. This is a qualitative test for investigating EV-D68 infection of humans. The test has identified both the original EV-D68 strains and contemporary strains which has been confirmed by subgenomic sequencing of partial 5'UTR-VP2 and partial VP1 sequencing. Culture of the virus is not required as this assay is capable of detecting EV-D68 direct from extracted clinical samples.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
hybrid