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https://doi.org/10.17504/proto...
Article . 2018 . Peer-reviewed
License: CC BY
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iGEM Registry Bacterial Transformation v1

descriptionPublicationkeyboard_double_arrow_right Article 16 Jul 2018Publisher:Springer Science and Business Media LLC
Authors: Vinoo not provided; Abigail not provided; Meagan not provided; Tiago Lubiana;

doi: 10.17504/protocols.io.rqsd5we

iGEM Registry Bacterial Transformation v1

Abstract

Protocol for heatshock transforming E.coli competent cells, from strains such as DH5-alpha. These can be made competent in-house or bought from various suppliers. Transformations are essential to using the DNA Distribution Kits: resuspend the DNA sample in a well, transform the DNA into competent cells, and select single colonies. However, transformations can also be one of the more fickle laboratory techniques. Read through the entire protocol before starting! If you have issues with transformations, read through the Troubleshooting page for help. (adapted from http://parts.igem.org/Help:Protocols/Transformation)

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
hybrid
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