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https://doi.org/10.17504/proto...
Article . 2018 . Peer-reviewed
License: CC BY
Data sources: Crossref
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Chromatin Immunoprecipitation v1

descriptionPublicationkeyboard_double_arrow_right Article 04 Jul 2018Publisher:ZappyLab, Inc.
Authors: Isabelle Georis;

doi: 10.17504/protocols.io.rhvd366

Chromatin Immunoprecipitation v1

Abstract

ChIP of yeast cells. Exponential phase growing cells are crosslinked, washed and stored at -80°C. Lysis is performed, unsoluble chromatin is isolated, with subsequend sonication of the chromatin to reach an average of 500bp fragments. IP is carried out using magnetic beads. Proteins are degraded, crosslinked is reversed and RNA is degraded. qPCR is performed on purified samples.

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    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 0
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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Powered by OpenAIRE graph
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
hybrid