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https://doi.org/10.17504/proto...
Article . 2018 . Peer-reviewed
License: CC BY
Data sources: Crossref
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Environmental DNA (eDNA) COI metabarcoding Illumina MiSeq NGS PCR Protocol v1

descriptionPublicationkeyboard_double_arrow_right Article 30 Jan 2018Publisher:Springer Science and Business Media LLC
Authors: Collin Closek; Anni Djurhuus; Katie Pitz; Ryan Kelly; Reiko Michisaki; Kristine Walz; Hilary Starks; +3 Authors

doi: 10.17504/protocols.io.mwnc7de

Environmental DNA (eDNA) COI metabarcoding Illumina MiSeq NGS PCR Protocol v1

Abstract

This protocol is aimed at amplifying the cytochrome c oxidase subunit I (COI) mitochondrial gene in eukaryotes. The primers (forward: mlCOIintF, reverse: HCO2198) utilized in this protocol are based on the primers utilized in Leray et al. 2013 (forward) and Folmer et al. 1994 (reverse). Amplicons generated using this protocol can then be sequenced using the Illumina platform. Primers used: Fluidigm CS1+mlCOIinfF Fluidigm CS2+HCO2198 Secondary COI PCR primers PE1-BC-CS1 PE2-BC-CS2

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 2
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
Powered by OpenAIRE graph
Found an issue? Give us feedback
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
2
Average
Average
Average
hybrid