CK-MB Detection Assay v1

Intended use: Kinetic system for determination of the isoenzyme MB of creatinine Kinase (CK-MB) activity in serum or plasma. Teste Principle: The serum is incubated with the work Reagent of CK-MB containing a specific antibody for the CK-MB subunit what completely inhibit the enzymatic activity of CK-MB monomer. The activity of CK-B monomer, which is not inhibited by the antibody, is measured by the fllowing ractions: Creatinine- Phosphate+ADP ---(CK-B) →Creatine +ATP ATP+ Glucose ---(HK)→ ADP+Glucose-6-Phosphate Glucose-6-Phosphate + NAD---(G-6-PDH)→ 6-PG + NADH

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