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https://doi.org/10.17504/proto...
Article . 2018 . Peer-reviewed
License: CC BY
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Environmental DNA (eDNA) 16S metabarcoding Illumina MiSeq NGS PCR Protocol v1

descriptionPublicationkeyboard_double_arrow_right Article 05 Feb 2018Publisher:Springer Science and Business Media LLC
Authors: Collin Closek; Anni Djurhuus; Katie Pitz; Ryan Kelly; Reiko Michisaki; Kristine Walz; Hilary Starks; +3 Authors

doi: 10.17504/protocols.io.m3ec8je

Environmental DNA (eDNA) 16S metabarcoding Illumina MiSeq NGS PCR Protocol v1

Abstract

This protocol is aimed at amplifying the 16S rRNA hypervariable region 4 (16S V4) in prokaryotes. The primers (515fBand 806rB, 926R) used in this protocol are based on the primer utilized in Apprill et al 2015 and the Earth Microbiome Project (EMP). CitationApprill, A., McNally, S., Parsons, R. & Weber, L. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquat Microb Ecol 75, 129–137 (2015). doi:10.3354/ame01753 Earth Microbiom Projecthttp://press.igsb.anl.gov/earthmicrobiome/protocols-and-standards/16s/

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    popularity
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
hybrid