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https://doi.org/10.17504/proto...
Article . 2016 . Peer-reviewed
License: CC BY
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RNA extraction using the 'home-made' Trizol substitute v1

descriptionPublicationkeyboard_double_arrow_right Article 10 Feb 2016Publisher:ZappyLab, Inc.
Authors: Matus Valach;

doi: 10.17504/protocols.io.eiebcbe

RNA extraction using the 'home-made' Trizol substitute v1

Abstract

Asimple protocol for RNA extraction from various types of samples (protists, fungi, bacteria, organelles, subcellular fractions, ribosomes, etc.). Compared tothe commercial Trizol® procedure, it makes use ofgenerally available chemicals without the need for columns,thusallowing concurrent extraction oftranscripts of all sizes (including RNAs <200 nt). Small circular DNA molecules (up to ~9 kbp) will also be efficiently isolated. The procedure is based on apreviously published protocol by Rodríguez-Ezpeleta et al. (DOI:10.1007/978-1-60327-136-3_3).

  • BIP!
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    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 5
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
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Found an issue? Give us feedback
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
5
Top 10%
Average
Average
hybrid