
In order to carry out the research project of genetic adaptation to high-altitude hypoxia in Sherpa highlanders, we recruited Sherpa highlanders in Namche Bazaar village at a high altitude of 3,440 meters (m) above sea level and non-Sherpa lowlanders in Kathmandu city at 1,300 m in Nepal. Venous blood was sampled to obtain plasma and extract DNA in each subject. The concentrations of factors in plasma were measured. The single-nucleotide polymorphisms (SNPs) in the hypoxia-associated genes were genotyped.
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