This protocol describes the preparation and staining of human FFPE material for multiplex visualization using Imaging Mass Cytometry (IMC). This protocol uses basic standard immunohistochemical staining techniques. The tissue is incubated with antibodies that have specific affinity for different cells and tissues in the context of a formalin fixed thin section of human tumor material. Antibodies are labeled with heavy metals instead of the typical fluorochromes, and are visualized using the Hyperion Imaging System manufactured by the Fluidigm corporation.