The following protocol was used for preparing dried blood spots in conjunction with serum samples for ELISA testing. Multiple plates were prepared and analyzed together. Elution of dried blood spots were prepared using published methods. Eluates serum samples and analyzed together. In this project varicella zoster, hepatitis A, tetanus toxoid and measles. These experiments were conducted for a project in which we hypothesized that DBS eluates and Serum specimens would correlate relatively when testing these specimens for selected viral antibodies using commercially prepared ELISA kits. With the goals of comparing the efficiency of dried blood spots and serum dilutions in the detection of IgG antibodies against: a) Measles, b)Tetanus, c)Varicella, d)Influenza and d) Hepatitis A. Secondarily, we aimed to determine the stability of the dried blood spot at relevant temperature changes and over time.