Human herpesvirus 6A (HHV-6A) is a T-lymphotropic betaherpesvirus that establishes lifelong latency in its host. Virus reactivations have been implicated in several diseases, including multiple sclerosis, encephalitis, myocarditis, and chronic fatigue syndrome, but HHV-6A biology remains poorly understood. Despite the availability of HHV-6A bacterial artificial chromosomes (BACs), it remains challenging to reconstitute the virus, hampering virological studies. Here, we describe an optimized protocol that enhances BAC transfection efficiency and accelerates virus propagation, facilitating virus reconstitution within two to three weeks. We used an HHV-6A GFP reporter virus, but the protocol also worked equally well for other HHV-6A BACs and mutants.