
This SOP describes the slide acquisition for spill-over correction of Imaging Mass Cytometry experiments. The protocol makes use of the compensation slide, which was prepared previously following the SOP: “HuBMAP_Protocol_3_Compensation_Slide_Preparation.pdf” to measure each conjugated antibody individually and to record all observed spill-over. The resulting compensation matrix can be used to compensate data that has been recorded using the same antibody conjugates.
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