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https://doi.org/10.17504/proto...
Article . 2020 . Peer-reviewed
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nCoV-2019 sequencing protocol for illumina v1

descriptionPublicationkeyboard_double_arrow_right Article 26 Mar 2020Publisher:Springer Science and Business Media LLC
Authors: Kentaro Itokawa; Tsuyoshi Sekizuka; Masanori Hashino; Rina Tanaka; Makoto Kuroda;

doi: 10.17504/protocols.io.bd9fi93n

nCoV-2019 sequencing protocol for illumina v1

Abstract

This protocol is folked from "ARTIC amplicon sequencing protocol for MinION for nCoV-2019" by Josh Quick to adapt it to illumina sequencers. Because the PCR products are fragmented and ligated with adapters, this protocol is not restricted to 250 PE mode of MiSeq. While the library preparation uses QiaSeq FX by Qiagen and is basically straight forward (as par kit instruction but set to 1/4 scale), some tweaks for much of simplicity and speed were added.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
2
Average
Average
Average
hybrid