Aquatic eDNA sampling and plant community metabarcoding with portable Nanopore Flongle sequencing (v0.0.3) v3
Aquatic eDNA sampling and plant community metabarcoding with portable Nanopore Flongle sequencing (v0.0.3) v3
Aquatic eDNA sampling, extraction, and plant metabarcoding on Oxford Nanopore's Flongle platform. Sampling method adapted from: Laramie, M.B., Pilliod, D.S., Goldberg, C.S. and Strickler, K.M., 2015. Environmental DNA sampling protocol-filtering water to capture DNA from aquatic organisms (No. 2-A13). US Geological Survey. https://pubs.er.usgs.gov/publication/tm2A13 Extraction protocol nearly identical to Qiagen DNEasy PowerWater kit manual: https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&ved=2ahUKEwj4jpSKkqfnAhUjnOAKHdAbDQ8QFjABegQIBBAB&url=https%3A%2F%2Fwww.qiagen.com%2Fus%2Fresources%2Fdownload.aspx%3Fid%3Dbb731482-874b-4241-8cf4-c15054e3a4bf%26lang%3Den&usg=AOvVaw32l8w94T6GAZUI4TAyUyT1 Amplicon sequencing is a modified version of the PCR barcoding protocol from Oxford Nanopore for kit SQK-PBK004.
- American Museum of Natural History United States
- Stonehill College United States
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