This SARS-CoV-2 targeted, and rapid viral RNA library preparation and sequencing kit provides an approximately five hour turn-around time from viral RNA sample to sequencing.The kit includes cDNA synthesis, enrichment, and library amplification and indexing.The enhanced protocol removes the need for normalization and quantification prior to amplification and indexing.RNA input is 5 ul, regardless of SC2 viral load in the sample extract.Amplification is performed using two primer pools that produces amplicons that are 300 bp in size, so no fragmentation is necessary prior to sequencing on Illumina platforms.10 bp unique dual indices are provided in the kit, and with a cycle read length of 149 for each paired-end read, the sequencing time is just over 28 hours.