Glycerol stocks v1
Igem Dusseldorf;
Glycerol stocks v1
Overnight liquid culture (3mL) + Antibiotic. For E.coli Low salt LB For Pichia YPD 80% Glycerol: mix 20mL H2O with 80mL pure glycerol and then autoclave E. coli: 812µL of cells mix with 188 µL of sterile 80% glycerol Pichia: Spin 2min at 2000g. Decant 2ml. Resuspend in remaining liquid. 812µL of cells mix with 188µL of sterile 80% glycerol. Use cryovials for freezing away.
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).0 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
selected citations
Citations provided by BIP!
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
Citations provided by BIP!popularityPopularity provided by BIP!
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
Popularity provided by BIP! 0
Average
Average
Average
hybrid
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