Purification Guide for the Isolation of Antibodies with ChroPack Columns by DALEX Biotech. With ChroPack Protein A/G columns you can quickly isolate monoclonal and polyclonal antibodies from various sample materials. Antibodies bind with high specificity to the recombinant protein G whose BSA binding site has been removed. The ChroPack columns can be operated with peristaltic pumps and common FPLC systems. The Luer-Lock connections make changing the column particularly easy. Each ml bed volume has a binding capacity of > 15 mg (tested with polyclonal human Ig, the binding capacity varies according to species and clones). Up to 98 % purity in a single purification step. No swelling/shrinking of the matrix in aqueous buffers. High thermal stability, up to 15 minutes at 80 °C in aqueous buffers with neutral pH. pH stability: short-term pH 2 - 8, long-term pH 3 - 8. Suitable for all common low-pressure systems.