In sediments from Chignahuapan Lake, State of Mexico, Mexico, 96 palynomorphs were identified. Eight taxa were found for algae, one family with one genus both for Equisetopsida and Lycopodiopsida, and 6 families with 10 genera for Polypodiopsida. Pinophyta were represented by 3 families and 3 genera, Liliopsida by 10 families and 13 genera, and Magnoliopsida by 44 families and 50 genera. The temperate forest surrounding Chignahuapan lake included components such as Abies, Alnus, Pinus, Quercus and Salix; aquatic and subaquatic vegetation components such as Cyperus, Luzula, Myriophyllum, Typha and Schoenoplectus were also present. Human activity was inferred from the occurrence of Zea mays and Cucurbitaceae.