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Brazilian Archives of Biology and Technology
Article . 2013 . Peer-reviewed
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Brazilian Archives of Biology and Technology
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Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species

Authors: Oliveira, Gilmara Alvarenga Fachardo; Pádua, Juliano Gomes; Costa, Juliana Leles; Jesus, Onildo Nunes de; Carvalho, Fabiana Moraes de; Oliveira, Eder Jorge de;

Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species

Abstract

The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be used for the establishment of molecular strategies for use in passion fruit breeding and genetics.

Keywords

Transferability, passion fruit, SSR, TP248.13-248.65, Biotechnology

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
14
Average
Average
Top 10%
Green
gold