In veterinary practice, tympanic temperature (TT) measurement is not very disseminated. It is surprising, because TT could have advantages compared to the rectal temperature (RT). In the present study, we investigate (Callithrix penicillata), right TT, left TT and RT. We used an infra-red thermometer to measure the temperature in marmosets of tympanic membrane, and a digital clinical thermometer for the measurement of rectal temperature. Right TT was lower than left TT and RT. There was not statistical difference between left TT and RT. Correlation between left TT and RT was stronger than correlation between right TT and both, left TT and RT. This results suggests of a regional thermal dissociation and an asymmetric cerebral metabolism and body temperature, due to the stress of contention in marmosets.