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Acta Biológica Colombiana
Article . 2014 . Peer-reviewed
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Acta Biológica Colombiana
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Acta Biológica Colombiana
Article . 2015
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ESTABLECIMIENTO DEL SISTEMA DE REGENERACION POR EMBRIOGÉNESIS SOMÁTICA DE Azadirachta indica A. Juss.

Authors: ARTIGAS R, Maria D; FERNANDEZ DA SILVA, Rafael;

ESTABLECIMIENTO DEL SISTEMA DE REGENERACION POR EMBRIOGÉNESIS SOMÁTICA DE Azadirachta indica A. Juss.

Abstract

<p><em>Azadirachta indica</em>, es una planta con múltiples aplicaciones tanto forestal como farmacológica. Por ende, el establecimiento del sistema de cultivo <em>in vitro</em> por embriogénesis somática ofrece diversas y variadas ventajas, tales como obtener plantas altamente productivas en metabolitos. En este estudio, se utilizaron secciones foliares y cotiledonares, inducidas en medios MS (1962) suplementados con: BAP sólo y combinado con ANA/2,4-D, TDZ sólo y con ABA. La regeneración fue con MS sólo o con K + AIA y BAP + AIA. Como resultado se estableció un sistema eficiente con secciones de cotiledones, observándose organogénesis a bajas concentraciones de BAP, mientras a altos niveles de BAP (2,5 mg.L<sup>-1</sup>), así como con TDZ + ABA (0,02 + 1mg.L<sup>-1</sup>) respectivamente favorecieron la embriogénesis somática primaria y secundaria en un 96 % y 71 % respectivamente. La regeneración fue 71 % con MS, mientras que el enraizamiento fue de 86,67 % con MS½, obteniéndose plantas completas a corto plazo. </p><p><strong>ABSTRACT</strong></p><p><em>Azadirachta indica</em>, is a plant with multiple forest and pharmacological application. Therefore, the establishment of <em>in vitro</em> culture system for somatic embryogenesis offers several distinct advantages such as obtaining highly productive plant metabolites. In this study, were used sections cotyledon and leaf, induced on MS medium (1962) supplemented with: BAP alone and combined with NAA / 2,4-D, TDZ alone and ABA. Regeneration was with MS alone or with K + BAP + IAA and IAA. As a result was established an efficient system with cotyledon sections, being observed organogenesis at low concentrations of BAP, while high levels of BAP (2.5 mg.L<sup>-1</sup>) with 96 % and TDZ + ABA (0.02 + 1mg.L<sup>-1</sup>) with 71 %, favoring the primary and secondary somatic embryogenesis. Regeneration was 71 % with MS; rooting was 86.67 % with MS½, presenting whole plants obtained short term.</p><p><strong>RESUMO</strong></p><p><em>Azadirachta indica, </em>é uma planta com várias aplicaçãos florestas e farmacológicas. Por conseguinte, a criação de sistema de cultur<em>a in vitro </em>para a embriogénese somática apresenta várias vantagens, tais como a obtenção de plantas altamente produtivas de metabolitos. Neste estudo, as seções de cotilédones e folhas, induzidas em meios suplementado en MS (1962) con: BAP sozinho e combinado com ANA / 2,4-D, TDZ e ABA sozinho. A regeneração foi apenas com MS ou com K + BAP + IAA e IAA. Como resultado, foi eficiente con seções cotiledones, observados organogênese em baixas concentrações de BAP, en quanto altos níveis de BAP (2,5 mg L-1) e com TDZ + ABA (0,02 + 1mg.L-1) favoreceu a embriogênese somática primária e secundária até 96 % e 71%, respectivamente. Regeneração estava com MS 71 %, en quanto que o enraizamento foi de 86,67 %, com ½ MS produzindo plantas inteiras curto prazo.</p><p><strong><br /></strong></p><p> </p>

Country
Colombia
Keywords

embriogénesis somática, 2,4-dichlorophenoxyacetic acid (2,4–D), QH301-705.5, ácido 2,4-diclorofenoxiacético (2,4-D), 57 Ciencias de la vida; Biología / Life sciences; biology, somatic embryogenesis, 6-Bencil-aminopurina (BAP), 6-Benzyl-aminopurine (BAP), regeneration, regeneración, Regeneration, Biology (General), 2,4-dichlorophenoxyacetic acid (2,4-D), Neem

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Average
Average
Average
Green
gold