The purpose of this study was to examine the reliability and validity of the Questionnaire on Stress in Diabetes Patients-Revised (QSD-R) and adapt it to the Turkish language for possible clinical use with diabetic patients in the Turkish Republic of Northern Cyprus (TRNC). A forward and backward translation was done by native speakers and professionals in the field. Translation consistency was reached and a pilot study confirmed that the level of comprehension of the Turkish version of the QSD-R. The study was conducted with 500 participants, over the age of 18 years of age with a confirmed diagnosis of diabetes at two state hospitals located in the TRNC. Twenty-two faulty questionnaires were removed and 478 questionnaires were examined. Multiple exploratory factor analyses using principal components analysis with varimax rotation resulted in the removal 7 items from the original questionnaire leaving a total of 38 items and yielded seven factors. Initial reliability analysis was found to be .939; however, after the items were removed, reliability analysis for the entire scale was found to be .843, which suggests that the QSD-R-TR is a reliable instrument. Validity for the QSD-R-TR was analyzed by conducting a discriminant validity analysis. QSD-R-TR subscale correlations were below <.70 which suggests that discriminant validity exists. Findings from this study suggest that the QSD-R-TR is a reliable and valid instrument that can be used in clinical practice with Turkish speaking diabetes patients in TRNC.