Inorganic Phosphate Deprivation Causes tRNA Nuclear Accumulation via Retrograde Transport inSaccharomyces cerevisiae
Copyright policy )Inorganic Phosphate Deprivation Causes tRNA Nuclear Accumulation via Retrograde Transport inSaccharomyces cerevisiae
AbstractNuclear export of tRNA is an essential eukaryotic function, yet the one known yeast tRNA nuclear exporter, Los1, is nonessential. Moreover recent studies have shown that tRNAs can move retrograde from the cytosol to the nucleus by an undefined process. Therefore, additional gene products involved in tRNA nucleus–cytosol dynamics have yet to be identified. Synthetic genetic array (SGA) analysis was employed to identify proteins involved in Los1-independent tRNA transport and in regulating tRNA nucleus–cytosol distribution. These studies uncovered synthetic interactions between los1Δ and pho88Δ involved in inorganic phopshate uptake. Further analysis revealed that inorganic phosphate deprivation causes transient, temperature-dependent nuclear accumulation of mature cytoplasmic tRNA within nuclei via a Mtr10- and retrograde-dependent pathway, providing a novel connection between tRNA subcellular dynamics and phosphate availability.
- The Ohio State University United States
- University of Toronto Canada
Cell Nucleus, Saccharomyces cerevisiae Proteins, Genotype, Biological Transport, RNA, Fungal, Saccharomyces cerevisiae, Polymorphism, Single Nucleotide, Phosphates, Mutagenesis, Insertional, Cytosol, RNA, Transfer, DNA, Fungal, Plasmids
Cell Nucleus, Saccharomyces cerevisiae Proteins, Genotype, Biological Transport, RNA, Fungal, Saccharomyces cerevisiae, Polymorphism, Single Nucleotide, Phosphates, Mutagenesis, Insertional, Cytosol, RNA, Transfer, DNA, Fungal, Plasmids
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