Abstract Through the application of basic forensic document examination principles, the known Josef Mengele handwriting from the period 1938 was examined and successfully compared to disputed handwriting made in 1949 and circa 1970s. These examinations resulted in the positive identification of Josef Mengele as the writer of the disputed material. Although these handwriting examinations did not confirm that Josef Mengele's remains were in Grave 321 at the cemetery at Embu, they verified the witness accounts of the last years of Dr. Josef Mengele's life.