Powered by OpenAIRE graph
Found an issue? Give us feedback
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Zeitschrift für Natu...arrow_drop_down
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
Zeitschrift für Naturforschung C
Article . 1979 . Peer-reviewed
License: CC BY NC ND
Data sources: Crossref
versions View all 2 versions
addClaim

This Research product is the result of merged Research products in OpenAIRE.

You have already added 0 works in your ORCID record related to the merged Research product.

A Viscosity Model of Polyacrylamide Gel Electrophoresis

Authors: Hans-Joachim Bode;

A Viscosity Model of Polyacrylamide Gel Electrophoresis

Abstract

In current theories of polyacrylamide gel electrophoresis, the idea prevails that molecular siev­ing relies on different accessibility of volume fractions and of cross-sectional area fractions (denot­ed “pores”) to different-sized ions due to the effect of “geometric exclusion”. This correlates with the assumption that all elements of a polyacrylamide network occupy fixed and unchangeable po­sitions thus forcing colliding macro-ions to diffuse laterally in order to find an “accessible pore” and to resume motion in direction of the electrical field. However, the alternative conception would be equally well justified, i. e. the assumption that polyacrylamide chains represent smooth obstacles cleared aside under the electrokinetic pressure of a macro-ion. This explanation would even be preferable with respect to the molecular sieving effects occuring in solutions of “liquid polyacrylamide”. Yet no theory exists as to describe such effects in quantitative terms. In the present article, a parameter is defined and discussed, which can be estimated by experi­ment, and which seems to be apt to characterize local resistivity of polymer structures against dis­location and deformation: the “fractional specific resistance”. Definition of this parameter is bas­ed on the model of a “viscosity-emulsion” composed of two interpenetrating liquid compartments which are characterized by different levels of hydrodynamic friction and the spatial dimensions of which are inferred from Ogston’s theory. This concept of “localized viscosity” may also serve as a link between theories of molecular sieving and of “macroscopic viscosity” of flexible polymers. The data of Morris, formerly taken as verifications of the “rigid-pore” concept, are now interpret­ed in terms of four factors responsible for sizediscrimination: collision frequency, duration of sin­gle contacts, size-dependent frictional force, and the extent of cooperation among fibres, due to crosslinking and to simultanous contacts of several fibres to a single macro-ion. Some functions rel­evant for problems of molecular weight determination by gel electrophoresis are discussed in rela­tion to the suggested model.

Keywords

Acrylamides, Viscosity, Molecular Conformation, Proteins, RNA, Sodium Dodecyl Sulfate, Electrophoresis, Polyacrylamide Gel, Mathematics

  • BIP!
    Impact byBIP!
    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    20
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
Powered by OpenAIRE graph
Found an issue? Give us feedback
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
20
Average
Top 10%
Average
hybrid