AbstractBased on impressionistic and acoustic data, Assamese is described as having a phonological tongue root harmony system, with blocking by certain phonological configurations and over-application in certain morphological contexts. This study explores physical properties of the patterns using ultrasonic imaging to determine whether the impressionistic descriptions match what speakers actually do. Principal components analysis (PCA) determines that most participants produce a contrast in tongue root position in the appropriate contexts, though there is less of an impact on tongue root with greater distance from the triggering vowel. Analysis uses the root mean squared distance (RMSD) calculation to determine whether both blocking and over-application take effect. The blocking results conform to the impressionistic descriptions. With over-application, [e] and [o] are expected; while some speakers clearly produce these vowels, others articulate a vowel that is indeterminant between the expected [e]/[o] and an unexpected [ɛ]/[ɔ]. No speaker consistently showed the expected tongue root position in all contexts, and some speakers appeared to have lost the contrast entirely, yet all are considered to be speakers of the same dialect of Assamese. Whether this (apparent) loss is a consequence of crude research methodologies or accurately reflects what is happening within the language community remains an open question.