Abstract Review of laboratory test data is a management focus for compliance laboratories. Using BERT-based intelligent analysis to analyze the laboratory instrument test data can assist laboratory testing personnel to quickly and accurately obtain the target data. This paper, with high-performance liquid chromatography (HPLC) as an example, applies big data technology and machine learning algorithm to the intelligent analysis and application of instrument test data by reading the output files in combination with the historical data of laboratory management system and business system, to realize the automatic acquisition of instrument test data, which reduces the laboratory labor cost, improves the laboratory test efficiency, optimizes the laboratory test management, and improves the quality control level.