descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1986Publisher:Walter de Gruyter GmbHJournal:Clinical Chemistry and Laboratory Medicine, volume 24 (issn: 1434-6621, eissn: 1437-4331,

Authors: Hoffmann, G.; Schmidt, D.; Bastian, B.; Guder, W.;

doi: 10.1515/cclm.1986.24.11.871

pmid: 2433381

Photometric Determination of Phospholipase A

- Summary
- Subjects
- Metrics

Abstract

A photometric method for the determination of phospholipase A is described, in which fatty acids are liberated from phosphatidylcholine and measured by a discontinuous enzymatic test. Optimal test conditions were evaluated using purified phospholipase A from human and porcine pancreas. The enzyme activities measured in normal human sera are in the range of 0 to 10 U/l. Pathological values reach more than 100 U/l. There is no correlation with the activities of lipase or amylase in cases of acute pancreatitis.

Related Organizations

Humboldt-Universität zu Berlin
Germany

Keywords

Swine, Lipase, Hydrogen-Ion Concentration, Phospholipases A, Photometry, Kinetics, Pancreatitis, Phospholipases, Amylases, Animals, Humans, Pancreas, Deoxycholic Acid

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	74
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%