Sequence determination of lychnin, a type 1 ribosome-inactivating protein from Lychnis chalcedonica seeds
Copyright policy )Sequence determination of lychnin, a type 1 ribosome-inactivating protein from Lychnis chalcedonica seeds
The complete amino acid sequence of lychnin, a type 1 ribosome-inactivating protein (RIP) isolated from Lychnis chalcedonica seeds, has been determined by automated Edman degradation and ESI-QTOF mass spectrometry. Lychnin consists of 234 amino acid residues with a molecular mass of 26 131.14 Da. All amino acid residues involved in the formation of the RIP active site (Tyr69, Tyr119, Glu170, Arg173 and Trp203) are fully conserved. Furthermore, a fast MALDI-TOF experiment showed that two out of three cysteinyl residues (Cys32 and Cys115) form a disulfide bridge, while Cys214 is in the thiol form, which makes it suitable for linking carrier molecules to generate immunotoxins and other conjugates.
Molecular Weight, Autoanalysis, Sequence Analysis, Protein, Molecular Sequence Data, Seeds, Lychnis, Ribosome Inactivating Proteins, Ribosome Inactivating Proteins, Type 1, Amino Acid Sequence, Edman degradation; Electrospray dual-ESI-sprayer system; Immunotoxin; Ribosome-inactivating protein;, Mass Spectrometry
Molecular Weight, Autoanalysis, Sequence Analysis, Protein, Molecular Sequence Data, Seeds, Lychnis, Ribosome Inactivating Proteins, Ribosome Inactivating Proteins, Type 1, Amino Acid Sequence, Edman degradation; Electrospray dual-ESI-sprayer system; Immunotoxin; Ribosome-inactivating protein;, Mass Spectrometry
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).18 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!