Tailor-Made Dyes for Fluorescence Correlation Spectroscopy (FCS)

descriptionPublicationkeyboard_double_arrow_right Article 21 Jan 2001Publisher:Walter de Gruyter GmbHJournal:Biological Chemistry, volume 382 (issn: 1431-6730,

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Authors: P, Czerney; F, Lehmann; M, Wenzel; V, Buschmann; A, Dietrich; G J, Mohr;

doi: 10.1515/bc.2001.062

pmid: 11347900

Tailor-Made Dyes for Fluorescence Correlation Spectroscopy (FCS)

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Abstract

Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes.

Keywords

Structure-Activity Relationship, Indoles, Spectrometry, Fluorescence, Lasers, Benzopyrans, Serum Albumin, Bovine, DNA, Fluorescent Dyes

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