The in vilro antibacterial and antifungal activities of tetrasubstituted benzene derivatives [1-chloro (or bromo or iodo)-2,4,6-trichloro and 1-chloro (or bromo or iodo)-2, 4,6-tribromo and 1-chloro (or bromo or iodo)-2, 4, 6-triiodo benzenes] derived from 2,4,6-trichloro tribromo and triiodo anilines were investigated. Tetrasubstituted benzene dervatives synthesized and identified by spectroscopic means IR and NMR. The antibacterial and antifungal activities were measured by Minumum inhibition concentration (MIC) method against gram-positive bacteria i.e. Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633; Gram-negative ones as Yersinia enterocolüica ATCC 1501 Escherichia coli ATCC 11230 and fungus as Candida albicans from our strain collection. Antimicrobial activies of these compounds tended to decrease with increasing size of halogene substitutents.