Pyrethrum (Tanacetum cinerariifolium), is plant species with the highest amount of natural insecticides - pyrethrins. An in-house information sys­ tem for the development of different plant tissue cultures and marketing of their products was designed. The application of the relational information system in set­ ting up a research hypothesis of high probability is discussed. By processing the information system, plant tissue cultures' parameters were identified, selected and modified. They were tested on the plant tissue culture of Tanacetum cinerariifoli­ um. The influence of jasmonic acid on axillary shoot differentiation was studied. An inhibitory effect of jasmonic acid on shoot tissue culture differentiation was proven (100 µM, 10 µM), and a prediction method for determination of the variable optimal concentration interval was presented (between 0,5 and 4,5 µM). In addition a HPLC method was introduced for pyrethrins determination.