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Acta Agriculturae Slovenica
Article . 2004 . Peer-reviewed
License: CC BY
Data sources: Crossref
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Acta Agriculturae Slovenica
Article . 2004
Data sources: DOAJ
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Razgradnja ksilana z različnimi ksilanazami vampne bakterije Pseudobutyrivibrio xylanivorans Mz5T

Authors: Čepeljnik, Tadej; Fidler, Katarina; Marinšek-Logar, Romana;

Razgradnja ksilana z različnimi ksilanazami vampne bakterije Pseudobutyrivibrio xylanivorans Mz5T

Abstract

Uporaba ksilanaz kot krmnih dodatkov je pri monogastričnih živalih zelo obetavna, saj so njihov ugoden učinek na zdravstveno stanje in prirast živali potrdile številne raziskave. Pred kratkim je bila opisana nova vrsta vampne bakterije P. xylanivorans s tipskim sevom Mz5T, ki je močno ksilanolitična. Nekaj njenih ksilanolitičnih encimov je že opisanih, preiskati pa želimo še ostale, da bi ugotovili vzrok visoke ksilanolitične aktivnosti in možnosti uporabe bakterije kot probiotika pri monogastričnih živalih (prašiči, perutnina) ali encimov kot krmnih dodatkov. Iz celičnega izvlečka smo uspeli delno očistiti ksilanazi, veliki 44 kDa in 81 kDa in delno proučiti njuno encimsko delovanje. Primerjali smo tudi encimsko delovanje na celično površino vezanih ksilanaz s ksilanazami celičnega izvlečka. 44 kDa ksilanaza je eksoksilanaza brez β-ksilozidazne aktivnosti, pri ksilanazi velikosti 81 kDa predvidevamo, da gre za endoksilanazo. Ksilanolitični encimi celične površine učinkovito razgrajujejo ksilan, vendar niso sposobni cepiti ksilobioze. Le-to razgradijo ksilanolitični encimi v notranjosti celice.

Country
Slovenia
Related Organizations
Keywords

rumen, Pseudobutyrivibrio xylanivorans, S, microbiology, mikrobiologija, pseudobutyrivibrio xylanivorans, Agriculture, anaerobic bacteria, xylan, info:eu-repo/classification/udc/579, anaerobne bakterije, ksilanaze, ksilan, xylanases, vamp

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green
Published in a Diamond OA journal