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Proteomic analysis in usual and nonspecific interstitial pneumonia.

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2014 Spain English Publisher:F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e HistologíaJournal:Histology and histopathology, volume 29 (issn: 1699-5848,

Authors: Ohara, Ichiyo; Aida, Shinsuke; Shimazaki, Hideyuki; Kobayashi, Hideo; Tsuda, Hitoshi; Toda, Tosifusa; Nakanishi, Kuniaki; +1 Authors

doi: 10.14670/hh-29.377

pmid: 24048960

handle: 10201/67123

Proteomic analysis in usual and nonspecific interstitial pneumonia.

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Abstract

Differentiating nonspecific interstitial pneumonia (NSIP) from usual interstitial pneumonia (UIP) is important for the determination of both treatment and prognosis. Using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), we examined 8 UIPs, 8 NSIPs, and 30 normal lung tissues. Comparisons with control in 2D-DIGE showed that (a) in UIP, nine protein spots were significantly upregulated and seven were significantly downregulated, (b) in NSIP, four protein spots were significantly upregulated and nine were significantly downregulated. The detected proteins were analyzed by MALDI-TOF mass spectrometry, allowing qualitative differences in vimentin subtypes to be characterized. One vimentin subtype was upregulated in UIP, while another one was downregulated in NSIP (vs. control). These different characteristics were partially supported by the results of Western blot analysis. Our immunohistochemistry revealed vimentin expression within fibroblasts (a) in fibroblastic foci in UIP and (b) in fibrotic alveolar walls in NSIP. Differences in vimentin subtypes may provide useful biomarkers for separating NSIP from UIP, alongside differences in histological characteristics.

Country

Spain

Related Organizations

National Defense Medical College
Japan
National Defense Medical College Hospital
Japan
University of Murcia
Spain
Tokyo Metropolitan Institute of Gerontology
Japan
Mita Hospital
Japan

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Keywords

Proteomics, Blotting, Western, Immunohistochemistry, Idiopathic Pulmonary Fibrosis, CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología, Diagnosis, Differential, :5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología [CDU], Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Humans, Vimentin, Electrophoresis, Gel, Two-Dimensional, Usual interstitial pneumonia, Lung Diseases, Interstitial, Biomarkers

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