the first European interuniversity center dedicated to promoting 3Rs principles in teaching and research was inaugurated in Pisa, Italy on March 14, 2018. The Centro 3R was spearheaded by the Universities of Pisa and Genoa. Membership is open to all Italian universities and agreements for twinning across Europe and other countries are being pursued. the Centro’s mission to promote rational and scientific thinking in experimental science through a multidisciplinary teaching and research approach inclusive of all 3Rs as a means to accelerate the R of replacement. The projects to develop an open resource-sharing web platform and interdisciplinary elective courses were also mentioned.