Babesia microti (Piroplasmida: Babesiidae) in nymphal Ixodes ricinus (Acari: Ixodidae) in the Czech Republic

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 2005Publisher:Biology Centre, AS CRJournal:Folia Parasitologica, volume 52, pages 274-276 (issn: 0015-5683, eissn: 1803-6465,

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Authors: Ivo, Rudolf; Maryna, Golovchenko; Silvie, Sikutová; Nataliia, Rudenko; Libor, Grubhoffer; Zdenek, Hubálek;

doi: 10.14411/fp.2005.036

pmid: 16270809

Babesia microti (Piroplasmida: Babesiidae) in nymphal Ixodes ricinus (Acari: Ixodidae) in the Czech Republic

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Abstract

A total of 350 nymphs of the common tick Ixodes ricinus (Linnaeus, 1758) were collected in an endemic focus of Lyme borreliosis (South Moravia, Czech Republic) and examined for the presence of the protozoan Babesia microti (França, 1909) by polymerase chain reaction (PCR), using primers specific for the B. microti gene encoding small subunit rRNA. The assay revealed five positive pools (out of 70 pools examined); the corresponding prevalence rate was about 1.5%. Sequence analysis of the PCR products confirmed their 100% homology with that of B. microti. The study represents the first evidence of B. microti in ixodid ticks in the Czech Republic.

Related Organizations

Biology Centre
Czech Republic
University of South Bohemia in České Budějovice
Czech Republic
Masaryk University
Czech Republic
Czech Academy of Sciences
Czech Republic

Keywords

Nymph, Ixodes, RNA, Ribosomal, Prevalence, Animals, Sequence Analysis, DNA, Babesia microti, Polymerase Chain Reaction, Czech Republic

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