An experimental investigation of the behavior of fiber reinforced concrete under cyclic flexural loading is presented. One type of polypropylene and two types of steel fibers in two different volume concentrations are studied. Load-deflection response is obtained for constant amplitude fatigue loading as well as for static loading. The damage level is recorded under static and fatigue loading using acoustic emission techniques. Data are presented in terms of complete load-deflection diagrams (for static loading) and in terms of S-N diagrams (for fatigue loading). Damage evolution is described in terms of acoustic emission activity as a function of deflection (static loading) or cycles (fatigue loading). The test results show that the addition of steel fibers increases the flexural fatigue strength considerably. Compared with plain concrete, the fatigue strength for 2 million cycles is changed from 60% to 90% of the ultimate flexure strength when the steel fiber content is one volume percent. High-fiber volume concentrations (2%) further increase absolute fatigue strength; however, fatigue performance measured relative to the static strength is decreased compared with the lower fiber volume concentration. Furthermore, the results show that the accumulated damage level at failure in the static test of unreinforced concrete is of the same order of magnitude as in the fatigue testing of the same material. However, using fiber reinforced concrete, the accumulated damage level in fatigue testing is 1-2 order of magnitude higher than the level reached in static testing of the same material. Finally, the tests show that the deflection at failure of the fiber reinforced concrete specimens under constant stress range fatigue loading can be predicted using the static load-deflection curve, provided the testing time is short enough to neglect creep effects.