The response of olive trees (Olea europaea L.) to a kaolin-based particle film formulation was evaluated in the warm sub-humid Mediterranean environment of northwest Syria for its effect on fruit yield, oil content and quality. Kaolin-sprayed olive trees significantly outperformed their unsprayed counterparts in terms of fruit yield and size, percentages of dry matter and oil content. Oil samples obtained from kaolin-sprayed trees contained less peroxides after 70 days of storage compared with that extracted from untreated control trees. Consistent with previous findings on apple and grapefruit trees, the kaolin particle film proved to be an efficient growth enhancer for olive trees in the sub-humid Mediterranean area.