Chloroplast Transformation
Chloroplast Transformation
In this chapter we briefly review the developmental history and current research status of chloroplast transformation and introduce the merits of chloroplast transformation as compared with the nuclear genome transformation. Furthermore, according to the chloroplast transformation achieved in oilseed rape (Brassica napus), we introduce the preparation of explants, transformation methods, system selection, identification methods of the transplastomic plants, and experimental results. The technical points, the bottleneck, and the further research directions of the chloroplast transformation are discussed in the notes.
- Institute of Genetics and Developmental Biology China (People's Republic of)
- Chinese Academy of Sciences China (People's Republic of)
Chloroplasts, Brassica rapa, Genetic Vectors, Cell Culture Techniques, Gene Transfer Techniques, Gene Expression, Plants, Genetically Modified, Plant Leaves, Blotting, Southern, Transformation, Genetic, Biological Assay
Chloroplasts, Brassica rapa, Genetic Vectors, Cell Culture Techniques, Gene Transfer Techniques, Gene Expression, Plants, Genetically Modified, Plant Leaves, Blotting, Southern, Transformation, Genetic, Biological Assay
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