Agrobacterium spp. are widely used to transform new genes into plant tissue. Transferring useful genes into bacteria in order to transform them into the target plant is an essential part of the process, which can be achieved by electroporation. Electroporation is a technique involving exposing cells to an electric field for a short duration, in order to facilitate the cross membrane transfer of material. This involves the transient production of pores or holes in the cell membrane. In order to gain an understanding of the cellular events caused by electroporation the following questions need to be answered:

Related Organizations

University of Dundee
United Kingdom
Abertay University
United Kingdom

Keywords

DNA, Bacterial, 572, DNA, Plant, Cell Membrane, Genetic Vectors, Gene Transfer Techniques, Transfection, Electroporation, Agrobacterium tumefaciens

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