
A notable shortcoming of the HPLC method for measuring 25-hydroxyvitamin D described by Lensmeyer et al. (1) is its failure to adequately remove interference by the C3-epimer. Although the authors dismiss this because the interference was less than the lowest limit of quantification, this would not be true in some samples collected from children >1 year old, in which the concentration of this metabolite can be as high as 92 μg/L(2 …
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