Introduction The use of portable gait measurement systems in research is appealing to collect real-world data at low-cost, low participant burden, and without requirement for dedicated lab space. Most commercially available inertial measurement units (IMU’s) designed for running only capture temporospatial data, the ability to capture biomechanics data such as shock and motion metrics with the RunScribe IMU makes it the closest to a lab alternative. The RunScribe system has been validated in running, however, is yet to be validated for walking. Method Qualisys motion capture, AMTI force plates, and Delsys Trigno accelerometers were used as gold standard lab measures for comparison against the RunScribe IMU. Twenty participants completed 10 footsteps per foot (20 total) measured by both systems simultaneously. Variables for validation included: Vertical Ground reaction force (GRF), instantaneous GRF rate, pronation excursion, pronation velocity, total shock, impact force, braking force. Interclass correlation (ICC) was used to determine agreement between the measurement systems, mean differences were used to evaluate group level accuracy. Results ICC results showed moderate agreement between measurement systems when both limbs were averaged. The greatest agreement was seen for GRF rate, pronation excursion, and pronation velocity (ICC = 0.627, 0.616, 0.539), low agreement was seen for GRF, total shock, impact shock, braking shock (ICC = 0.269, 0.351, 0.244, 0.180). However mean differences show the greatest level of accuracy for GRF, GRF rate, and impact shock. Discussion Results show mixed agreement between the RunScribe and gold standard lab measures, and varied agreement across left and right limbs. Kinematic variables showed the greatest agreement, however GRF had the lowest relative mean difference for group results. The results show acceptable levels of agreement for most variables, however further work must be done to assess the repeatability and sensitivity of the RunScribe to be applied within areas such as footwear testing and gait retraining protocols.