Actin Cytoskeleton Regulates Hippo Signaling
Copyright policy )Actin Cytoskeleton Regulates Hippo Signaling
Hippo pathway controls the organ size by modulating cell proliferation and apoptosis. However, the upstream regulation of hippo signaling by actin cytoskeleton is not clear. To elucidate the role of actin as an upstream regulator of Hippo signaling, the levels of F (filamentous)-actin in cells were elevated using jasplakinolide, an actin-stabilizing drug. Induction of F-actin formation in HeLa cells resulted in decreased phosphorylation of YAP, a key effector molecule for Hippo signaling. The activated YAP is localized to the cell nucleus and YAP increase was associated with increased expression of downstream CCN growth factors CCN1/CYR61 and CCN2/CTGF. The effect of the actin-stabilizing drug was blocked when YAP levels were suppressed in YAP "knock-down" cells. In summary, using an actin-stabilizing drug we show that actin cytoskeleton is one of the upstream regulators of Hippo signaling capable of activating YAP and increasing its downstream CCN growth factors.
- Stanford University United States
- Stanford University School of Medicine United States
- Salk Institute for Biological Studies United States
Science, Immunoblotting, Gene Expression, Cell Cycle Proteins, Protein Serine-Threonine Kinases, Polymerization, Depsipeptides, Humans, Hippo Signaling Pathway, Phosphorylation, Reverse Transcriptase Polymerase Chain Reaction, Q, R, Connective Tissue Growth Factor, Nuclear Proteins, Actins, Actin Cytoskeleton, Medicine, RNA Interference, Research Article, Cysteine-Rich Protein 61, HeLa Cells, Signal Transduction, Transcription Factors
Science, Immunoblotting, Gene Expression, Cell Cycle Proteins, Protein Serine-Threonine Kinases, Polymerization, Depsipeptides, Humans, Hippo Signaling Pathway, Phosphorylation, Reverse Transcriptase Polymerase Chain Reaction, Q, R, Connective Tissue Growth Factor, Nuclear Proteins, Actins, Actin Cytoskeleton, Medicine, RNA Interference, Research Article, Cysteine-Rich Protein 61, HeLa Cells, Signal Transduction, Transcription Factors
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