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Imaging of Surfaces by Concurrent Surface Plasmon Resonance and Surface Plasmon Resonance-Enhanced Fluorescence

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 25 Mar 2010 English Publisher:Public Library of Science (PLoS)Journal:PLoS ONE, volume 5, page e9833 (eissn: 1932-6203,

Authors: Paul Yager; Rahber Thariani;

doi: 10.1371/journal.pone.0009833

pmid: 20360841

pmc: PMC2845608

Imaging of Surfaces by Concurrent Surface Plasmon Resonance and Surface Plasmon Resonance-Enhanced Fluorescence

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Abstract

Surface plasmon resonance imaging and surface plasmon induced fluorescent are sensitive tools for surface analysis. However, existing instruments in this area have provided limited capability for concurrent detection, and may be large and expensive. We demonstrate a highly cost-effective system capable of concurrent surface plasmon resonance microscopy (SPRM) and surface plasmon resonance-enhanced fluorescence (SPRF) imaging, allowing for simultaneous monitoring of reflectivity and fluorescence from discrete spatial regions. The instrument allows for high performance imaging and quantitative measurements with surface plasmon resonance, and surface plasmon induced fluorescence, with inexpensive off-the-shelf components.

Related Organizations

University of Rome Tor Vergata
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Washington State University
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University of Mary
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University of Washington
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University of Washington
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Keywords

Light, Surface Properties, Science, Cost-Benefit Analysis, Q, R, Equipment Design, Surface Plasmon Resonance, Microscopy, Fluorescence, Calibration, Medicine, Scattering, Radiation, Biological Assay, Research Article

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