
This paper presents a microscopic imaging technique that uses variable-angle illumination to recover the complex polarimetric properties of a specimen at high resolution and over a large field-of-view. The approach extends Fourier ptychography, which is a synthetic aperture-based imaging approach to improve resolution with phaseless measurements, to additionally account for the vectorial nature of light. After images are acquired using a standard microscope outfitted with an LED illumination array and two polarizers, our vectorial Fourier ptychography (vFP) algorithm solves for the complex 2x2 Jones matrix of the anisotropic specimen of interest at each resolved spatial location. We introduce a new sequential Gauss-Newton-based solver that additionally jointly estimates and removes polarization-dependent imaging system aberrations. We demonstrate effective vFP performance by generating large-area (29 mm2), high-resolution (1.24 μm full-pitch) reconstructions of sample absorption, phase, orientation, diattenuation, and retardance for a variety of calibration samples and biological specimens.
Biological Physics (physics.bio-ph), Image and Video Processing (eess.IV), FOS: Electrical engineering, electronic engineering, information engineering, FOS: Physical sciences, Physics - Biological Physics, Electrical Engineering and Systems Science - Image and Video Processing, Physics - Optics, Optics (physics.optics)
Biological Physics (physics.bio-ph), Image and Video Processing (eess.IV), FOS: Electrical engineering, electronic engineering, information engineering, FOS: Physical sciences, Physics - Biological Physics, Electrical Engineering and Systems Science - Image and Video Processing, Physics - Optics, Optics (physics.optics)
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