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Biomedical Optics Express
Article . 2021 . Peer-reviewed
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Biomedical Optics Express
Article
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PubMed Central
Article . 2021
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https://dx.doi.org/10.48550/ar...
Article . 2020
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Method for assessing the spatiotemporal resolution of structured illumination microscopy (SIM)

Authors: Boualam, A; Rowlands, C;

Method for assessing the spatiotemporal resolution of structured illumination microscopy (SIM)

Abstract

A method is proposed for assessing the temporal resolution of structured illumination microscopy (SIM), by tracking the amplitude of different spatial frequency components over time, and comparing them to a temporally-oscillating ground-truth. This method is used to gain insight into the performance limits of SIM, along with alternative reconstruction techniques (termed ‘rolling SIM’) that claim to improve temporal resolution. Results show that the temporal resolution of SIM varies considerably between low and high spatial frequencies, and that, despite being used in several high profile papers and commercial microscope software, rolling SIM provides no increase in temporal resolution over conventional SIM.

Country
United Kingdom
Related Organizations
Keywords

570, 0205 Optical Physics, Image and Video Processing (eess.IV), FOS: Physical sciences, Electrical Engineering and Systems Science - Image and Video Processing, Article, 620, FOS: Electrical engineering, electronic engineering, information engineering, eess.IV, physics.optics, 0912 Materials Engineering, Physics - Optics, Optics (physics.optics)

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    popularity
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    influence
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    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
6
Top 10%
Average
Top 10%
Green
gold