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Laboratory Medicine
Article . 2006 . Peer-reviewed
Data sources: Crossref
Laboratory Medicine
Article . 2008 . Peer-reviewed
Data sources: Crossref
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Principles of Immunochemical Techniques Used in Clinical Laboratories

Authors: Richard L. Krogsrud; Marja E. Koivunen;

Principles of Immunochemical Techniques Used in Clinical Laboratories

Abstract

Immunochemistry offers simple, rapid, robust yet sensitive, and in most cases, easily automated methods applicable to routine analyses in clinical laboratories. Immunochemical methods do not usually require extensive and destructive sample preparation or expensive instrumentation. In fact, most methods are based on simple photo-, fluoro-, or luminometric detection. Immunochemical methods have rapidly replaced chromatographic techniques in clinical diagnostics, offering fast detection of antibodies associated with specific diseases, disease biomarkers, hormones, and pharmaceuticals. The assays most often used in clinical immunochemistry involve either quantitative or qualitative formats using enzymelinked immunosorbent assays (ELISAs), immunochromatography in the form of lateral-flow devices like dip-sticks and test strips or Western Blot assays used to interpret data from protein analysis with gel electrophoresis. Similarly, immunohistochemistry, one of the main diagnostics tools in today’s clinical laboratories, is also based on the principles of antigen-antibody binding.

  • BIP!
    Impact byBIP!
    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    66
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
66
Top 10%
Top 10%
Average
bronze