Abstract. Hossain MM, Uddin MS, Baral PK, Ferdus M, Bhowmik S. 2022. Phytochemical screening and antioxidant activity of Ipomoea hederifolia stems: A potential medicinal plant. Asian J Nat Prod Biochem 20: 41-47. Ipomoea hederifolia L., a plant of Convolvulaceae family, popularly known as morning glory, possesses numerous medicinal values. The present study aimed to explore the antioxidant activity and bioactive compounds of I. hederifolia stems (IHS). The IHS was soaked in methanol for 21 days. The filtrate was concentrated using rotary evaporator after filtration to obtain IHS extract which is subjected to phytochemical screening using various tests. Antioxidant activity was determined using 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and reducing power activity (RPA) methods. The phytochemical screenings revealed that IHS extract possesses carbohydrates, tannins, flavonoids, phenols, saponin, alkaloids, steroids, anthraquinones, and cardiac glycosides. The antioxidant activity of IHS extract in DPPH model was moderate (IC50 :174.08 µg/ml) in comparison with standards (IC50: 102.28 µg/mL AA; IC50: 88.52 µg/mL for BHT). It indicates that IHS exerts free radical scavenging power in dose dependent fashion. The RPA model also produced moderate antioxidant potential (EC50: 279.58 µg/mL for IHS; EC50: 23.121 µg/mL for AA; EC50: 50.84 µg/mL for BHT) depending on increasing order of dose. Based on the findings of this investigation, we can conclude that IHS extract possesses various bioactive compounds and moderate antioxidant potentials which may be path to discovery of traditional medicines and remedies for many critical diseases.