A ground water catchment was instrumented as a karst hydrology and water quality laboratory to develop long-term flow and water quality data. This catchment located in Woodford and Jessamine Counties in the Inner Bluegrass, Central Kentucky encompasses approximately 1620 ha, 40 water wells, over 400 sinkholes, 2 karst windows, and 1 sinking stream. The land uses consist of approximately 59% beef pasture, horse farm, and golf course; 16% row crops; 6% orchard; 13% forest; and 6% residential. The instrumentation consisted of a recording rain gage, an H-flume, a water stage recorder, and an automated water sampler. Flow data for 312 days were analyzed, and a peak flow rate prediction equation, specific to this catchment, was developed. Recession curves were analyzed and found to be of two distinct mathematical forms, log curves and exponential curves. Prediction equations were good for the log-type recession curve and fair for the exponential-type recession curve. For the exponential recessions, the peak flow rate was found to be bimodally distributed. The recession events were classified as either high flow or low flow, with the point of separation at 113 L/s. It was hypothesized that the flow system was controlled by pipe flow above 113 L/s and by open channel flow below 113 L/s. Subsequent analysis resulted in adequate prediction for the low flow events. Explained variation associated with the high flow events was low and attributed to storage in the karst system that was not incorporated into the predictor equation.