Monitoring Starch Saccharification Using Low Field NMR
Monitoring Starch Saccharification Using Low Field NMR
The present study is an attempt to develop a low field NMR based technique for fast analysis of enzymatic saccharification of corn starch. Experiments were first conducted on model samples containing pure starch and glucose to prove the hypothesis. Starch saccharification was carried out using two commercial enzymes, and monitored using CPMG NMR pulse sequence using a low field NMR system (0.5 T, RI Maran DRX NMR Imaging System). NMR-determined and conventional method-determined glucose concentrations were compared. Data obtained using the CPMG pulse sequence were fitted to a multi-component model. Good linear correlations between NMR relaxation parameters (relaxation time and amplitude) and concentrations of starch and glucose were observed.
- University of Minnesota United States
- University of Minnesota System United States
- University of Tennessee at Knoxville United States
- Nanchang University China (People's Republic of)
- United States Department of the Army United States
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